Second, acid sphingomyelinase like phosphodiesterase 3b encoded by smpdl3b has a role in the conversion of sphingomyelin to ceramide and its levels are reduced in renal biopsy samples from. Csbel021850hu for the quantitative determination of human acid sphingomyelinaselike phosphodiesterase 3b smpdl3b concentrations in serum, plasma, tissue homogenates, cell lysates. Has in vitro phosphodiesterase activity, but the physiological substrate is unknown pubmed. Sphingomyelin phosphodiesterase acidlike 3a smpdl3a is one of three members of the acid sphingomyelinase asmaselike enzyme family, which also comprises the founding member asmase encoded by smpd1 and sphingomyelin phosphodiesterase acidlike 3b smpdl3b encoded by smpdl3b. The lipidmodulating enzyme sphingomyelin phosphodiesterase acidlike 3b smpdl3b is a key determinant of podocyte injury and a known off target of the anticd20 antibody rituximab rtx.
During dc maturation, crosspresentation is first upregulated and then turned off. Rituximab therapy for focal segmental glomerulosclerosis. Fine epitope mapping of humanized antiige monoclonal. Comparative proteomic analysis of rat hepatic stellate. Annotation program, chordata protein annotation program.
Sphingomyelin phosphodiesterase acidlike 3b smpdl3b. Sphingomyelinaselike phosphodiesterase 3b expression levels determine. Exploring the influence of the surface proteins on probiotic. Sphingomyelinaselike phosphodiesterase 3b expression. In order to understand the mediation function of surface proteins in probiotic effects executed by lactobacillus pentosus hc2 in midgut of litopenaeus vannamei, the immune and digestion related enzymes and the transcriptome expression were analyzed after shrimp fed with normal hc2 or with stripped surface proteins hc2 by lithium chloride licl treatment. Research article open access rituximab therapy for focal segmental glomerulosclerosis and minimal change disease in adults. Longterm adaptations in hepatic gene expression are important for complete understanding of liver function. Although the other three genes neuromodulin, acid sphingomyelinase like phosphodiesterase, and frizzled6 also were differentially expressed in cerebral vasospasm, the functional significance of these molecules currently is unknown. Identification of their epitope reveals the structural basis.
Smpdl3b elisa kit sphingomyelin phosphodiesterase, acidlike 3b. Complete information for smpdl3b gene protein coding, sphingomyelin phosphodiesterase acid like 3b, including. Smpdl3b sphingomyelin phosphodiesterase, acidlike 3b. In this study, after longterm dka exposure to zebrafish danio rerio, 47 protein spots had greater than 2fold differential expression as compared to the control. Smpdl3b is a gpianchored sm phosphodiesterase with relevance for cellular lipid composition. A biochemical characterization of rsasml does not support a. Human endomysium em antibody igm elisa kit cusabio. Oct 11, 2016 the structure and catalytic mechanism of human sphingomyelin phosphodiesterase like 3a an acid sphingomyelinase homolog with a novel nucleotide hydrolase activity. T1 sphingomyelinaselike phosphodiesterase 3b mediates radiationinduced damage of renal podocytes. Second, acid sphingomyelinaselike phosphodiesterase 3b encoded by smpdl3b has a role in the conversion of sphingomyelin to ceramide and its levels are reduced in renal biopsy samples from. Acid sphingomyelinaselike phosphodiesterase 3b uniprot. Furthermore we have recently demonstrated an important role of a sphingolipid related enzymes acid sphingomyelinaselike phosphodiesterase 3b, smpdl3b as modulators of podocyte function, which are kidney cells heavily affected in diabetes. Use of rituximab rtx for focal segmental glomerulosclerosis fsgs and minimal change disease mcd is widely described in children.
Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity pubmed. Antisphingomyelin phosphodiesterase acid like 3b antibody. Dec 15, 2015 during dc maturation, crosspresentation is first upregulated and then turned off. Us patent application for method of using cyclodextrin. Crystal structure of the acid sphingomyelinaselike. Ipr017064 acid sphingomyelinaselike phosphodiesterase, predicted. Identification of their epitope reveals the structural. Generation of biologically active linear and cyclic peptides has revealed a unique fine specificity of rituximab and its possible crossreactivity with acid sphingomyelinase like phosphodiesterase 3b precursor. Acid sphingomyelinaselike phosphodiesterase 3b smpdl3b is a protein with. Human acid sphingomyelinaselike phosphodiesterase 3b. Although a potential substrate for smpdl3b is sphingomyelin, we identify other.
Refinement software 7 phenix 117 refmac 90 cns 36 xplor 11 buster 6 shelxl97 2 xtalview. The enzyme acid sphingomyelinaselike phosphodiesterase 3b. Sphingomyelin phosphodiesterase acid like 3b smpdl3b affects insulin signaling in podocytes. Genes that were upregulated were vascular endothelial growth factor, glucoseregulated protein 78, growtharrest and dnadamageinducible protein, neuromodulin, protein disulfide isomeraserelated protein p5, acid sphingomyelinase like phosphodiesterase, and five genes related to inflammation monocyte chemotactic protein 1, cystatin b, inter. Human acid sphingomyelinase like phosphodiesterase 3b smpdl3b elisa kit catalog number. Frontiers chronic psychosocial stress in mice is associated. Human acid sphingomyelinase structures provide insight to. Sphingomyelin phosphodiesterase acid like 3a smpdl3a is one of three members of the acid sphingomyelinase asmase like enzyme family, which also comprises the founding member asmase encoded by smpd1 and sphingomyelin phosphodiesterase acid like 3b smpdl3b encoded by smpdl3b. Acts as a negative regulator of tolllike receptor signaling by similarity 455 aa. Comparative analysis between endometrial proteomes of. Variants of this antigen may also be found in other species, including canine, porcine, monkey, mouse and rat. Rituximab and therapeutic plasma exchange in recurrent focal. Generation of biologically active linear and cyclic peptides has revealed a unique fine specificity of rituximab and its possible crossreactivity with acid sphingomyelinaselike phosphodiesterase 3b precursor.
Sphingomyelinaselike phosphodiesterase 3b mgi symbol. T1 sphingomyelinase like phosphodiesterase 3b mediates radiationinduced damage of renal podocytes. The enzyme acid sphingomyelinase like phosphodiesterase 3b smpdl3b was shown to act as a negative regulator of innate immune signaling, affecting cellular lipid composition and membrane fluidity. We have solved the structure of smpdl3a revealing a calcineurin like fold.
Acts as a negative regulator of tolllike receptor signaling by similarity. Diseases associated with smpdl3b include lipoid nephrosis. Smpdl3b elisa kit sphingomyelin phosphodiesterase, acid. Smpdl3b acid sphingomyelinase like phosphodiesterase 3b, unc45a protein unc45 homolog a, ugdh udpglucose 6dehydrogenase, ifit1 interferoninduced protein with tetratricopeptide repeats 3, gale udpgalactose4epimerase. Comparative proteomic analysis of rat hepatic stellate cell. In a subset of patients with limited cutaneous lc systemic sclerosis ssc, anticenpa antibodies ab crossreact with a peptide foxe3p5362 that presents striking homology with one of the two immunodominant epitopes of cenpa ap1730. The current study investigates the role of sphingolipids in radiationinduced podocytopathy.
In ipa analysis, the statistical data were generated by the software. For example, cd40 autoantibodies have been implicated in modifying the effect of supar in focal segmental glomerulosclerosis, 33 whereas levels of acid sphingomyelinase like phosphodiesterase 3b. Acts as a negative regulator of tolllike receptor signaling pubmed. Exploring the influence of the surface proteins on. Smpdl3b, sphingomyelin phosphodiesterase acid like 3b orthology source. Acid sphingomyelinase like phosphodiesterase 3b smpdl3b, microtubuleactin crosslinking factor 1 macf1, putative glycerophosphodiester phosphodiesterase 5 gde5. In addition, we detected six proteins that were specifically expressed in the endometrial c area of pregnant ewes. Sphingomyelinaselike phosphodiesterase 3b expression levels determine podocyte injury phenotypes in glomerular disease. Murine acid sphingomyelinaselike phosphodiesterase 3b. Smpdl3b acid sphingomyelinaselike phosphodiesterase 3b, unc45a protein unc45 homolog a, ugdh udpglucose 6dehydrogenase, ifit1 interferoninduced protein with tetratricopeptide repeats 3, gale udpgalactose4epimerase. Sphingomyelinaselike phosphodiesterase 3b mediates.
Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and. Identification of genes differentially expressed in canine. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Smpdl3a acid sphingomyelinaselike phosphodiesterase 3a. Jun 01, 2015 the dried scx peptide fractions were resuspended in 80. For example, cd40 autoantibodies have been implicated in modifying the effect of supar in focal segmental glomerulosclerosis, 33 whereas levels of acid sphingomyelinaselike phosphodiesterase 3b. Gene ontology go annotations related to this gene include hydrolase activity and sphingomyelin phosphodiesterase activity. Smpdl3b acid sphingomyelinaselike phosphodiesterase 3b. Acid sphingomyelinaselike phosphodiesterase 3b 2 dna repair and telomere maintenance protein nbs1 2 nad 5nucleotidase 2. Csbel021850hu for the quantitative determination of human acid sphingomyelinase like phosphodiesterase 3b smpdl3b concentrations in serum, plasma, tissue homogenates, cell lysates. The density of specific bands was analyzed using the imagequant software program and standardized as the ratio of the density of pthr1 to the density of.
Smpdl3b sphingomyelin phosphodiesterase acid like 3b is a protein coding gene. Similarly, glycosphingolipids accumulate in the kidney of diabetic rats. Sphingomyelinaselike phosphodiesterase 3bmediates radiationinduced damage of renal podocytes. Gene references into functions our results identify the membranemodulating enzyme smpdl3b as a negative regulator of tlr signaling that functions at the interface of membrane biology and innate immunity. The protein may also be known as asml3b, acid sphingomyelinaselike phosphodiesterase 3b, and asmlike phosphodiesterase 3b. We have solved the structure of smpdl3a revealing a calcineurinlike fold. Identification and biochemical characterization of an acid. Human acid sphingomyelinaselike phosphodiesterase 3bsmpdl3b. Acts as a negative regulator of toll like receptor signaling by similarity 455 aa.
Recombinant human acid sphingomyelinaselike phosphodiesterase 3bsmpdl3b yeastcsbyp835664hu licsbep835664hu baculoviruscsbbp835664hu mammalian cellcsbmp835664hu in vivo biotinylation in licsbep835664hub. Human acid sphingomyelinaselike phosphodiesterase 3b smpdl3b elisa kit catalog number. This study was supported, in part, by the intramural research program of the. Although the other three genes neuromodulin, acid sphingomyelinaselike phosphodiesterase, and frizzled6 also were differentially expressed in cerebral vasospasm, the functional significance of these molecules currently is unknown. Liver metabolism and health in dairy cows during the periparturient period are affected by plane of nutrition prepartum. Located on chromosome 1, this gene encodes for acid sphingomyelinase like phosphodiesterase 3b precursor protein. Oct 16, 2018 sphingomyelinase like phosphodiesterase 3b mgi symbol. Acid sphingomyelinaselike phosphodiesterase 3b smpdl3b, microtubuleactin crosslinking factor 1 macf1. Effects of adjuvants for human use in systemic lupus. Smpdl3b elisa kit sphingomyelin phosphodiesterase, acidlike 3b smpdl3b elisa kit sphingomyelin phosphodiesterase, acidlike 3b. Pdf sphingomyelinphosphodiesteraseacidlike3b affects.
Human sphingomyelinase phosphodiesterase like 3a smpdl3a is a secreted enzyme that shares a conserved catalytic domain with human acid sphingomyelinase asmase, the enzyme carrying mutations causative of niemannpick disease. In vivo, inhibition of acid sphingomyelinase reduced proteinuria in. Rituximab therapy for focal segmental glomerulosclerosis and. The structure and catalytic mechanism of human sphingomyelin phosphodiesterase like 3a an acid sphingomyelinase homolog with a novel nucleotide hydrolase activity. Dammaccogeneration of biologically active linear and cyclic peptides has revealed a unique fine specificity of rituximab and its possible crossreactivity with acid sphingomyelinaselike phosphodiesterase 3b precursor. Diketone antibiotics dkas can produce chronic toxicity in aquatic ecosystems due to their pseudopersistent in the environment. We searched for clinical correlates of antifoxe3p5362 ab by measuring their levels along with those of ab to ap1730 and to the second. Apr 25, 2015 in addition, we detected six proteins that were specifically expressed in the endometrial c area of pregnant ewes.
Diabetes and obesity research program, research programs unit. Here we identify rsasml, previously annotated as rsp1609. Nglycosylation of human sphingomyelin phosphodiesterase. Tested in western blot wb and immunohistochemistry ihc applications. Smpdl3b modulates insulin receptor signaling in diabetic kidney. The objective of this study was to determine the treatment outcomes of rtx in adults with fsgs and mcd. Furthermore, several reports identified this enzyme as an off target of the therapeutic antibody rituximab, with implications in kidney disorders. Sphingomyelinaselike phosphodiesterase 3b mediates radiation. The dried scx peptide fractions were resuspended in 80. Located on chromosome 1, this gene encodes for acid sphingomyelinaselike phosphodiesterase 3b precursor protein. Smpdl3b elisa kit sphingomyelin phosphodiesterase, acidlike 3b details for product smpdl3b elisa kit no.
Sphingomyelin phosphodiesterase acidlike 3b smpdl3b affects insulin signaling in podocytes. Yoo th, pedigo ce, guzman j, correamedina m, wei c, villarreal r, mitrofanova a, leclercq f, faul c, li j, kretzler m, nelson rg, lehto m, forsblom c, groop ph, reiser j, burke gw, fornoni a, merscher s. Upregulation of parathyroid hormone receptor in cerebral. Rituximab and therapeutic plasma exchange in recurrent. Nglycosylation of human sphingomyelin phosphodiesterase acid. Acid sphingomyelinaselike phosphodiesterase 3a 8 sphingomyelin phosphodiesterase 8. Smpdl3b gene genecards asm3b protein asm3b antibody. Amigorena and colleagues show now that during a transient phase after tlr4 engagement, which lasts up to 18 hr, dc maturation results in the topological reorganization of lysosomes by rab34. This decreases phagolysosome fusion and strongly enhances crosspresentation efficiency. The enzyme acid sphingomyelinaselike phosphodiesterase 3b smpdl3b was shown to act as a negative regulator of innate immune signaling, affecting cellular lipid composition and membrane fluidity. This antibody reacts with bovine, canine, equine, guinea pig, human, mouse, rabbit, rat, zebrafish samples. Sphingomyelin phosphodiesterase acidlike 3b smpdl3b is a lipid raft. Dammaccogeneration of biologically active linear and cyclic peptides has revealed a unique fine specificity of rituximab and its possible crossreactivity with acid sphingomyelinase like phosphodiesterase 3b precursor. Soluble urokinase receptor and chronic kidney disease nejm.